Recent trends in sorbents for bioaffinity chromatography

Kip C., HAMALOĞLU K. Ö., Demir C., Tuncel A.

JOURNAL OF SEPARATION SCIENCE, cilt.44, sa.6, ss.1273-1291, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 44 Sayı: 6
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/jssc.202001117
  • Dergi Adı: JOURNAL OF SEPARATION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.1273-1291
  • Anahtar Kelimeler: boronate affinity chromatography, cell affinity capture, immobilized metal affinity chromatography, metal oxide affinity chromatography, protein A affinity chromatography, AFFINITY-CHROMATOGRAPHY, ENRICHMENT
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.