Investigation of lichen based green synthesis of silver nanoparticles with response surface methodology

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YILDIZ N., Ates C., Yilmaz M., Demir D., YILDIZ A., ÇALIMLI A.

GREEN PROCESSING AND SYNTHESIS, vol.3, no.4, pp.259-270, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 3 Issue: 4
  • Publication Date: 2014
  • Doi Number: 10.1515/gps-2014-0024
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.259-270
  • Hacettepe University Affiliated: Yes


In this study, silver (Ag) nanoparticles were successfully biosynthesized from AgNO3 through a simple green route using the lichen extract [Cetraria islandica (L) Ach] as a reducing and stabilizing agent. The mean sizes of spherical Ag nanoparticles with diameters ranging from 5 nm to 29 nm were obtained at different reaction conditions. The nanoparticles were characterized using transmission electron microscopy (TEM), energy dispersive X-ray (EDX), ultraviolet-visible (UV-VIS) and Fourier transform infrared spectroscopy (FTIR) techniques. Response surface methodology (RSM) was used to investigate the effects of temperature, reaction time and AgNO3/lichen ratio on green synthesis of Ag nanoparticles. The results showed that the increase in reaction time and AgNO3/lichen ratio caused a decrease of particle size and the increase in temperature resulted in bigger particles.