Akademik Veri Yönetim Sistemi
Journal of Drug Delivery Science and Technology, cilt.91, 2024 (SCI-Expanded)
The aim of this study is to design a drug delivery system in which both the carrier material and the flavonoid anticancer, chemopreventive and chemotherapeutic active substance to be loaded into this material are biologically sourced soft material. In this study, bovine serum albumin nanoparticles (BSANPs) were synthesized using “Desolvation Method”. Characterization studies were carried out by performing SEM, TEM, FT-IR, XRD, TGA, DSC and Zetasizer analyses. The most suitable BSA concentration was determined to be 25 mg/ml, and the size of BSANPs was measured at 156.9 nm with a PDI value of 0.078 in a medium with pH adjusted to 10 after 24 h of cross-linking. The optimum ratio of distilled water (solvent) to ethanol (non-solvent) for desolvation was found to be 1:2. The maximum CUR encapsulation efficiency and capacity of BSANPs were obtained as 90.2 % and 44.22 %, respectively, at a cross-linking time of 24 h using 15 mg of CUR and 110 μl of glutaraldehyde. The cumulative CUR release percentage from CUR-BSANPs containing 0.5, 1.0 and 1.5 mg CUR was determined to be 51.6 %, 67.3 % and 56.2 %, respectively, after 72–168 h in pH 5.6 PBS buffer. The conjugation of folic acid to CUR-BSANPs slightly reduced the release rate and the cumulative percentage release of CUR, but still provided a sustained and controlled release of CUR. In order to analyze the CUR release kinetic mechanism, the fit of CUR release profiles to zero order, first order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas models was examined. The values of kinetic constants for each model were calculated.