Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum

Andac M., Galaev I. Y., DENİZLİ A.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.109, ss.259-265, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 109
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.colsurfb.2013.03.054
  • Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.259-265
  • Anahtar Kelimeler: Albumin depletion, Cryogels, Protein imprinting, Proteome applications, ION-EXCHANGE, AFFINITY, CHROMATOGRAPHY, POLYMERS, RECOGNITION, HEMOGLOBIN, BIOMARKERS, ANTIBODIES, PROTEINS, CHITOSAN
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Macroporous cryogels imprinted with human serum albumin (HSA) have been prepared by copolymerization of 2-hydroxyethyl methacrylate with a functional co-monomer of N-methacryloyl-L-phenylalanine. The cryogels were used for the depletion of HSA from human serum. HSA-imprinted cryogels were prepared with gel fraction yields up to 90%, and their chemical structure, morphology and porosity were characterized by FTIR-spectroscopy, scanning electron microscopy, swelling studies and flow dynamics. Selective binding experiments were performed in the presence of competitive proteins like human transferrin and myoglobin. Albumin-imprinted cryogel column was optimized for fast protein liquid chromatography. Sodium-dodecyl sulfate polyacrylamide gel electrophoresis was used to show the efficiency of albumin depletion. (C) 2013 Elsevier B.V. All rights reserved.