Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum

Andac M., Galaev I. Y., DENİZLİ A.

COLLOIDS AND SURFACES B-BIOINTERFACES, vol.109, pp.259-265, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 109
  • Publication Date: 2013
  • Doi Number: 10.1016/j.colsurfb.2013.03.054
  • Journal Name: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.259-265
  • Keywords: Albumin depletion, Cryogels, Protein imprinting, Proteome applications, ION-EXCHANGE, AFFINITY, CHROMATOGRAPHY, POLYMERS, RECOGNITION, HEMOGLOBIN, BIOMARKERS, ANTIBODIES, PROTEINS, CHITOSAN
  • Hacettepe University Affiliated: Yes

Abstract

Macroporous cryogels imprinted with human serum albumin (HSA) have been prepared by copolymerization of 2-hydroxyethyl methacrylate with a functional co-monomer of N-methacryloyl-L-phenylalanine. The cryogels were used for the depletion of HSA from human serum. HSA-imprinted cryogels were prepared with gel fraction yields up to 90%, and their chemical structure, morphology and porosity were characterized by FTIR-spectroscopy, scanning electron microscopy, swelling studies and flow dynamics. Selective binding experiments were performed in the presence of competitive proteins like human transferrin and myoglobin. Albumin-imprinted cryogel column was optimized for fast protein liquid chromatography. Sodium-dodecyl sulfate polyacrylamide gel electrophoresis was used to show the efficiency of albumin depletion. (C) 2013 Elsevier B.V. All rights reserved.