Bone Marrow Mesenchymal Stem Cells from Donors with a High BMI Display ER Stress and Are Functionally Impaired.


Ulum B., Teker H. T. , Sarıkaya A., BALTA G. , KUŞKONMAZ B. B. , ÇETİNKAYA F. D. , ...More

JOURNAL OF CELLULAR PHYSIOLOGY, vol.2018, pp.1-8, 2018 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Letter
  • Volume: 2018
  • Publication Date: 2018
  • Title of Journal : JOURNAL OF CELLULAR PHYSIOLOGY
  • Page Numbers: pp.1-8

Abstract

Bone marrow mesenchymal stem cells (BMMSCs) are promising candidates for

regenerative medicine purposes. The effect of obesity on the function of BMMSCs is

currently un known. Here, we assessed how obesity affects the function of BMMSCs and

the role of endoplasmic reticulum ( ER) stress and the unfolded prote in response (UPR)

therein. BMMSCs were obtained from healthy donors with a normal (<25) or high (>30)

body mass index (BMI ). HighBMI BMMSCs displayed severely impaired osteogenic and

diminished adipogenic differentiation, decreased proliferation rates, increased senes-

cence, and elevated expression of ER stressrelated genes ATF4 and CHOP. Suppression

of ER stress using taurourso deoxycholic acid (TUDCA) and 4phenylbutyrate (4PBA)

resulted in partial recovery of osteogenic differentiation capacity, with a significant

increase in the expression of ALPL and improvement in the UPR. These data indicate that

BMI is important du ring the selection of BMMSC donors for regen erative medicine

purposes and that application of highBMI BMMSCs with TUDCA or 4PBA may

improve stem cell function. However, whether this improvement can be translated into

an in vivo clinical advantage remains to be assessed.