Immobilization of laccase on hairy polymer grafted zeolite particles: Degradation of a model dye and product analysis with MALDI-ToF-MS


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ÇELİKBIÇAK Ö. , Bayramoglu G., Yilmaz M., Ersoy G., Bicak N., SALİH B. , ...Daha Fazla

MICROPOROUS AND MESOPOROUS MATERIALS, cilt.199, ss.57-65, 2014 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 199
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.micromeso.2014.08.003
  • Dergi Adı: MICROPOROUS AND MESOPOROUS MATERIALS
  • Sayfa Sayıları: ss.57-65

Özet

Poly(2-chloroethyl acrylate), p(CEA), as a functional polymer was grafted via surface initiated atom transfer radical polymerization (SI-ATRP) on zeolite particles. The p(CEA) grafted zeolite particles were aminated, and a ligninolytic enzyme "laccase" from Trametes versicolor was covalently immobilized on the composite particles via glutaraldehyde coupling. The amount of immobilized laccase on the zeolite-g-p(CEA) particles was determined as 17.7 mg g(-1) particles. The degradation of a model dye (i.e., Reactive Red 120; RR-120) with the free and immobilized laccases was evaluated in batch and continuous systems. The covalently immobilized laccase showed a high decolorization efficiency toward RR-120 in aqueous solution compared to the free counterpart. The resulting dye degradation products were analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI ToF-MS). (C) 2014 Elsevier Inc. All rights reserved.

Poly(2-chloroethyl acrylate), p(CEA), as a functional polymer was grafted via surface initiated atom transfer radical polymerization (SI-ATRP) on zeolite particles. The p(CEA) grafted zeolite particles were aminated, and a ligninolytic enzyme “laccase” from Trametes versicolor was covalently immobilized on the composite particles via glutaraldehyde coupling. The amount of immobilized laccase on the zeolite-g-p(CEA) particles was determined as 17.7 mg g−1 particles. The degradation of a model dye (i.e., Reactive Red 120; RR-120) with the free and immobilized laccases was evaluated in batch and continuous systems. The covalently immobilized laccase showed a high decolorization efficiency toward RR-120 in aqueous solution compared to the free counterpart. The resulting dye degradation products were analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI–ToF-MS).