The preparation of chitosan membrane improved with nanoparticles based on unsaturated fatty acid for using in cancer-related infections


Kizaloglu A., Kilicay E., KARAHALİLOĞLU Z., Hazer B., DENKBAŞ E. B.

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, cilt.35, ss.328-350, 2020 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 35
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1177/0883911520943222
  • Dergi Adı: JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
  • Sayfa Sayıları: ss.328-350

Özet

This study includes the design of a chitosan membrane decorated with unsaturated fatty acid-based carrier system for cancer treatment and antibacterial application. For this, polystyrene-graft-polyoleic acid-graft-polyethylene glycol was prepared by free radical polymerization and characterized. Nanoparticles and caffeic acid-loaded nanoparticles were prepared by solvent evaporation technique and optimized. The short-term stability of nanoparticles was investigated at 4 degrees C. Drug encapsulation and loading efficiency were evaluated. The chitosan membrane and caffeic acid-loaded nanoparticles embedded into chitosan membrane were fabricated. The caffeic acid loaded nanoparticles embedded into chitosan membrane showed controlled release. The mechanical properties of all samples were investigated. The caffeic acid-loaded nanoparticles embedded into chitosan membranes indicated excellent antibacterial properties against theEscherichia coliandStaphylococcus aureus. The anticancer activity of all the samples was evaluated against SaOS-2 human primary osteogenic sarcoma and MC3T3-E1 pre-osteoblast cell lines by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, the flow cytometry and double staining methods. As a result, the designed carrier system showed great potential to cancer-associated infections treatment in bone cancer cases.