Candidate Angiogenesis‐Related Biomarkers in Patients with Laryngeal Carcinoma (AngLaC): A Prospective Cohort Study

Pamuk A. E., Gedik M. E., Sutay Suslu N., Gunaydin G.

OTOLARYNGOLOGY - HEAD AND NECK SURGERY, vol.00, no.00, pp.1-10, 2023 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 00 Issue: 00
  • Publication Date: 2023
  • Doi Number: 10.1002/ohn.219
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1-10
  • Hacettepe University Affiliated: Yes


Objective. Angiogenesis is indeed a vital process in the

progression of carcinomas, including that of larynx. Therefore,

this study (AngLaC) aimed to identify candidate angiogenesisrelated

biomarkers in laryngeal carcinoma patients.

Study Design. Prospective controlled cohort study.

Setting. Tertiary referral center.

Methods. In silico analyses of angiogenesis-related genes in

laryngeal carcinoma were performed to determine candidate

biomarkers. Serum levels of candidate biomarkers were

determined via enzyme-linked immunosorbent assay in

laryngeal carcinoma patients as well as in an age and

gender-matched control group. The associations of the

biomarkers with clinical parameters were investigated.

Results. The study included 60 laryngeal carcinoma patients

and 20 healthy controls. The serum levels of osteopontin,

IGFBP-3, VEGF, sVEGFR-1, and VEGFR-2 were significantly

higher in the patient group (p < .001, p ≤ .001, p < .001,

p < .01, p < .01, respectively). High osteopontin and sVEGFR-

1 levels were associated with locoregional-recurrence

(p = .024, p = .016, respectively). IGFBP-3 had the highest

diagnostic sensitivity (81.4%) and specificity (80%) among the

molecules that were investigated (p < .001). High sVEGFR-1

and low VEGFR-2 levels were associated with poor overallsurvival

(p = .037, p = .027, respectively). High osteopontin

and sVEGFR-1 levels were associated with poor diseasespecific

survival rates (p = .035, p = .018, respectively).

Conclusion. High serum levels of sVEGFR-1 and osteopontin

as well as low serum levels of VEGFR-2 proved to be poor

prognostic in terms of survival in laryngeal carcinoma. VEGF,

sVEGFR1, VEGFR2, IGFBP-3, and osteopontin levels were

found to be significantly increased in larynx cancer patients

compared to the normal population. Further studies on

osteopontin and sVEGFR-1 are required in order to

determine their associations with recurrence