Biofunctionalization of cellulosic fibres with L-cysteine: Assessment of antibacterial properties and mechanism of action against Staphylococcus aureus and Klebsiella pneumoniae

Caldeira E., Piskin E. , Granadeiro L., Silva F., Gouveia I. C.

JOURNAL OF BIOTECHNOLOGY, vol.168, no.4, pp.426-435, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 168 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1016/j.jbiotec.2013.10.021
  • Page Numbers: pp.426-435


The main purpose of this work is to obtain a cotton-based textile material functionalized with L-cysteine (L-cys) to achieve an antimicrobial effect with potential application in biomedical, geriatric or pediatric textiles. The binding capacity of L-cys to cotton fibres was assessed through different functionalization strategies surface activation and exhaustion processes. A subsequent analysis of the possible antibacterial action against Staphylococcus aureus and Klebsiella pneumoniae was performed according with the Japanese International standard (JISL, 2008). To determine the mechanism of action of L-cys on the selected strains, flow cytometry was used.