RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods

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Selvam K., Najib M. A., Khalid M. F., Mohamad S., Palaz F., Ozsoz M., ...More

DIAGNOSTICS, vol.11, no.9, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 11 Issue: 9
  • Publication Date: 2021
  • Doi Number: 10.3390/diagnostics11091646
  • Journal Name: DIAGNOSTICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, INSPEC, Directory of Open Access Journals
  • Keywords: COVID-19, SARS-CoV-2, RT-LAMP, CRISPR, Cas12, Cas13, NUCLEIC-ACID DETECTION, AMPLIFICATION
  • Hacettepe University Affiliated: Yes

Abstract

Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has attracted public attention. The gold standard for diagnosing COVID-19 is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). However, RT-qPCR can only be performed in centralized laboratories due to the requirement for advanced laboratory equipment and qualified workers. In the last decade, clustered regularly interspaced short palindromic repeats (CRISPR) technology has shown considerable promise in the development of rapid, highly sensitive, and specific molecular diagnostic methods that do not require complicated instrumentation. During the current COVID-19 pandemic, there has been growing interest in using CRISPR-based diagnostic techniques to develop rapid and accurate assays for detecting SARS-CoV-2. In this work, we review and summarize reverse-transcription loop-mediated isothermal amplification (RT-LAMP) CRISPR-based diagnostic techniques for detecting SARS-CoV-2.