PHEMA cryogel for in-vitro removal of anti-dsDNA antibodies from SLE plasma

ÖZGÜR E., BERELİ N., TÜRKMEN D., ÜNAL S., DENİZLİ A.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol.31, no.5, pp.915-920, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.1016/j.msec.2011.02.012
  • Journal Name: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.915-920
  • Keywords: Antibody removal, Anti-dsDNA, PHEMA, Cryogels, DNA, SLE, IMMOBILIZED POLYHYDROXYETHYLMETHACRYLATE MICROBEADS, DNA ANTIBODIES, CHOLESTEROL REMOVAL, AFFINITY SORPTION, IMMUNOADSORPTION, BEADS, AUTOANTIBODIES, PATIENT, IGG, CHROMATOGRAPHY
  • Hacettepe University Affiliated: Yes

Abstract

Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA) cryogel carrying DNA was used in the removal of anti-dsDNA antibodies from systemic lupus erythematosus (SLE) patient plasma. The PHEMA cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomer frozen inside a plastic syringe. After thawing, the PHEMA cryogel contains a continuous matrix having interconnected macropores of 10-200 mu m size. Pore volume in the PHEMA cryogel was 67.5%. Ester groups in the PHEMA structure were converted to imine groups by reacting with poly(ethyleneimine) (PEI) in the presence of NaHCO3. Amino (-NH2) content of PEI-modified PHEMA cryogel was determined as 82 mg PEI/g. Then, DNA was attached onto the PHEMA cryogel via amino groups (53.4 mg DNA/g cryogel). Anti-dsDNA-antibody concentration declined significantly from 780 IU/ml to 80 IU/ml with the time. The maximum anti-dsDNA-antibody adsorption amount was 70 x 10(3) IU/g. Anti-dsDNA-antibodies could be repeatedly adsorbed and eluted without noticeable loss in the anti-dsDNA-antibody adsorption amount. (C) 2011 Elsevier B.V. All rights reserved.