In our age, there are some qualifications of universal criteria that individuals should have to be able to survive in different societies with their knowledge, ability and skills. The concept of mathematical literacy which is the subject of this study is emphasized in recent years to be one of the important qualifications that individuals should have. In this study it is aimed to investigate mathematical literacy self-efficacy beliefs of pre-service teachers in terms of their department, graduated high school, grade level and gender. Data were collected with "Student Information Form" which is formed by researchers and "Mathematical Literacy Self-Efficacy Scale" which is developed by Ozgen and Bindak (2008). Data analysis shows that self-efficacy beliefs of biology, chemistry and mathematics pre-service teachers differ in favor of mathematics pre-service teachers and self-efficacy beliefs between biology and physics pre-service teachers differs in favor of physics pre-service teachers. When the type of graduated high school is considered, there is a significant difference between Anatolian Teacher High School and Technical High School in favor of the former. Furthermore, according to the grade level and gender variables, there is a significant difference in favor of fifth class and males.