Oak honey is a honeydew type of honey. The aim of this research was to investigate the characteristic features of Turkish oak honey and to obtain new results about oak honey. For this purpose, oak honey samples were collected from regions, where Quercus frainetto Ten. was found and oak honey was produced intensely, between June 2016 and 2017. Melissopalynological analysis, total phenolic and flavonoid content analysis, sugar analysis and chemical analysis were conducted for oak honey characterization. Total phenolic contents were found to be between 134.71 +/- 0.05 and 422.01 +/- 0.07 mgGAE/kg; total flavonoid contents were found to be between 31.84 +/- 0.09 and 59.25 +/- 0.23 mgQE/kg in Quercus frainetto honeydew honey samples. Fructose to glucose ratios were found to be between 1.01 and 1.23. GC-MS chemical analyses of honey samples revealed the presence of alcohols, acids, aliphatic acids and their esters, aldehydes, aromatic aldehydes, aromatic hydrocarbons, carboxylic acids and their esters, flavonoids, ketones, terpenes and other chemical compounds. To the best of our knowledge, this is the most comprehensive study on Turkish oak honey thus far.