Molecular and Biochemical Analysis of Durum Wheat Genotypes to Examine Carotenoid Pigment Content and Lipoxygenase Enzyme Activity


SÖNMEZOĞLU Ö. A. , Balkan A. S.

CEREAL RESEARCH COMMUNICATIONS, cilt.42, ss.218-228, 2014 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 42 Konu: 2
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1556/crc.2013.0070
  • Dergi Adı: CEREAL RESEARCH COMMUNICATIONS
  • Sayfa Sayıları: ss.218-228

Özet

The main criterion that determines the quality of durum wheat is the degree of suitability for pasta production (pasta-processing quality). In this regard, pigment content and the quantity of oxidative enzymes of durum wheat play important roles in the quality of pasta. It is now possible to examine these features and specify their effects using recently developed genetic markers and spectrophotometric measurement techniques. In the present study, LOX enzyme activity and pigment content are determined using molecular and biochemical scanning. According to the obtained results, Gediz-75, Gdem-12, Line-19, Zenit, Line-7 and Line-20 were determined as the most suitable lines or varieties for the production of quality pasta with regard to LOX enzyme activity. As for pigment content, Kyle, Zenit, Gdem-12, Gdem-2, TMB-1 and TMB-3 showed the highest potential for the production of yellow pasta. When pigment content and LOX enzyme activity were evaluated together, the potential of the Gediz-75, Gdem-12 and Zenit durum wheat varieties and lines to produce yellow pasta products was shown to be very high.