Ion-imprinted beads for molecular recognition based mercury removal from human serum


Andac M., Mirel S., Senel S., Say R., Ersoz A., Denizli A.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.40, no.2, pp.159-166, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 2
  • Publication Date: 2007
  • Doi Number: 10.1016/j.ijbiomac.2006.07.002
  • Journal Name: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.159-166
  • Keywords: molecular imprinting, molecular recognition, mercury removal, metal detoxification, affinity binding, SOLID-PHASE EXTRACTION, HEAVY-METAL IONS, AQUEOUS-SOLUTIONS, HUMAN PLASMA, PRECONCENTRATION, POLYMERS, CADMIUM, SEPARATION, EXPOSURE, SPECTRA
  • Hacettepe University Affiliated: Yes

Abstract

The aim of this study is to prepare ion-imprinted polymers which can be used for the selective removal of mercury ions [Hg2+] from human serum. N-Methacryloyl-(L)-cysteine (MAC) was chosen as the complexing monomer. In the first step, Hg2+ was complexed with MAC and the Hg2+-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-cysteine) (MIP) beads were synthesized by suspension polymerization. After that. the template ions (i.e., Hg2+) were removed using thiourea (0.5%, v/v) in 0.05 M HCl. The specific surface area of the MIP beads was found to be 59.04 m(2)/g with a size range of 63-140 mu m in diameter and the swelling ratio was 91.5%. According to the elemental analysis results, the MIP beads contained 87.0 mu mol MAC/g polymer. The maximum adsorption capacity was 0.45 mg Hg2+/g beads. The applicability of two kinetic models including pseudo-first order and pseudo-second order model was estimated on the basis of comparative analysis of the corresponding rate parameters, equilibrium capacity and correlation coefficients. Results suggest that chemisorption processes could be the rate-limiting step in the adsorption process. The relative selectivity coefficients of MIP beads for Hg2+/Cd2+, Hg2+/Zn2+ were 14.7 and 21.5 times greater than the non-imprinted (NIP) matrix. respectively. The MIP beads could be used many times without decreasing in their adsorption capacities significantly. (c) 2006 Elsevier B.V. All rights reserved.