Measurement of in vitro phagocytic activity using functional groups carrying monodisperse poly(glycidyl methacrylate) microspheres in rat blood


Ulusoy A., Onur M.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.14, no.11, pp.1299-1310, 2003 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 11
  • Publication Date: 2003
  • Doi Number: 10.1163/156856203322553509
  • Journal Name: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1299-1310

Abstract

Phagocytic activity of monocyte and neutrophil leukocytes in rat blood was investigated C using monodisperse poly(glycidyl methacrylate) (poly(GMA)) microspheres. Interactions of monocyte and neutrophil leukocytes with plain poly(GMA), charged poly(GMA)-NH2 and biomodified poly(GMA) (poly(GMA)-albumin and opsonized poly(GMA)) was studied as a function of time. Here we report the cellular phagocytosis rate of particles and the total internalised particle amount. Phagocytes showed higher phagocytic activity for poly(GMA) microspheres carrying functional groups versus plain poly(GMA) particles. Phagocytic activity was the highest for opsonized poly(GMA) microspheres (P < 0.05).