The aim of this study was to determine the concentration of residual monomers and to evaluate the effectiveness of elimination methods of residual monomers in three different fissure sealant materials (Helioseal F, Filtek Flow and EXM-510). The sealant materials were divided into four subgroups because of the treatment methods used; one control group and three experimental groups (cotton roll, rubber cup and prophylaxis paste in cup). High performance liquid chromatography was used to determine the concentrations of residual monomers. Results of the study showed that residual Bis-glycidyl dimethacrylate elution was the highest in Helioseal F and the lowest in Filtek Flow with the three methods tested. For triethleneglycol dimethacrylate, EXM-510 eluted the highest residual monomer. It was also found that although the three tested methods were insufficient for removing all of the residual monomers and rubbing with cotton rolls was more effective than other two methods.