Simultaneous determination of rosiglitazone and metformin in plasma by gradient liquid chromatography with UV detection

Yardimci C., Ozaltin N., Gurlek A.

TALANTA, vol.72, no.4, pp.1416-1422, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 72 Issue: 4
  • Publication Date: 2007
  • Doi Number: 10.1016/j.talanta.2007.01.042
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1416-1422
  • Hacettepe University Affiliated: Yes


A novel, fast and simple liquid chromatographic method was developed and validated for the simultaneous determination of rosiglitazone and metformin in human plasma. The analysis was performed on a phenyl column (250 mm x 4.6 mm i.d., 5 mu m) using a gradient method starting with mobile phase composed of acetonitrile:5 mM acetate buffer pH 5.5 (75:25, v/v). The flow rate was 1 mL min(-1). UV detection was performed at 245 nm and verapamil was used as internal standard. The total run time was less than 10 min. Sample preparation included a simple protein precipitation step with acetonitrile. Validation experiments were performed to demonstrate stability, specificity, sensitivity, linearity, accuracy, precision and robustness. The limit of quantification was 100 ng mL(-1) for rosiglitazone and 250 ng mL(-1) for metformin. The extraction recoveries were 100.02-105.0% for rosiglitazone, and 105.64-103.88% for metformin. The method was applied with success to plasma samples obtained from diabetic patients undergoing treatment with rosiglitazone and metformin. (C) 2007 Elsevier B.V. All rights reserved.