Dual Responsive Disposable Electrode for the Enumeration ofEscherichia coliin Whole Blood


Panhwar S., İLHAN H., Hassan S. S. , Zengin A., BOYACI İ. H. , TAMER U.

ELECTROANALYSIS, 2020 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2020
  • Doi Number: 10.1002/elan.202060185
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts

Abstract

In this report, we have designed a dual responsive disposable electrode for the enumeration ofEscherichia coli K12 (E. coli K12). The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal-organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro-electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantifyE. coliwith a ranging from 10(1)to 10(7) cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.