Dual Responsive Disposable Electrode for the Enumeration ofEscherichia coliin Whole Blood
ELECTROANALYSIS, vol.32, no.10, pp.2244-2252, 2020 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 32 Issue: 10
- Publication Date: 2020
- Doi Number: 10.1002/elan.202060185
- Journal Name: ELECTROANALYSIS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
- Page Numbers: pp.2244-2252
- Keywords: Disposable electrode, spectro-electrochemical labels, Cyclic Voltammetry, Pathogens bacteria, blood, GOLD NANOPARTICLES, ELECTROCHEMICAL DETECTION, ESCHERICHIA-COLI, E. COLI, BIOSENSOR, BACTERIA, O157H7, SENSOR
- Hacettepe University Affiliated: Yes
Abstract
In this report, we have designed a dual responsive disposable electrode for the enumeration ofEscherichia coli K12 (E. coli K12). The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal-organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro-electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantifyE. coliwith a ranging from 10(1)to 10(7) cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.