Dual Responsive Disposable Electrode for the Enumeration ofEscherichia coliin Whole Blood

Panhwar S., İLHAN H., Hassan S. S., Zengin A., BOYACI İ. H., TAMER U.

ELECTROANALYSIS, vol.32, no.10, pp.2244-2252, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 10
  • Publication Date: 2020
  • Doi Number: 10.1002/elan.202060185
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.2244-2252
  • Keywords: Disposable electrode, spectro-electrochemical labels, Cyclic Voltammetry, Pathogens bacteria, blood, GOLD NANOPARTICLES, ELECTROCHEMICAL DETECTION, ESCHERICHIA-COLI, E. COLI, BIOSENSOR, BACTERIA, O157H7, SENSOR
  • Hacettepe University Affiliated: Yes

Abstract

In this report, we have designed a dual responsive disposable electrode for the enumeration ofEscherichia coli K12 (E. coli K12). The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal-organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro-electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantifyE. coliwith a ranging from 10(1)to 10(7) cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.