Characterization of redox polymer based electrode and electrochemical behavior for DNA detection

KURALAY F., ERDEM GÜRSAN K. A., ABACI S., Ozyoruk H., Yildiz A.

ANALYTICA CHIMICA ACTA, vol.643, pp.83-89, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 643
  • Publication Date: 2009
  • Doi Number: 10.1016/j.aca.2009.03.051
  • Journal Name: ANALYTICA CHIMICA ACTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.83-89
  • Hacettepe University Affiliated: Yes

Abstract

Characterization of redox polymer, poly(vinylferrocenium) perchlorate (PVF+ClO4-) coated as a film on Pt electrodes, and the detection of DNA based on the electrochemical behavior of the polymer were described in this study. PVF+ClO4- Modified electrodes were prepared by the electrooxidation of poly(vinylferrocene) (PVF), and DNA immobilized polymer modified electrode was then prepared. The characterization of the polymer modified electrodes were performed by scanning tunneling Microscopy (STM), Raman spectroscopy and alternating current (AC) impedance. The effects of DNA concentration. pH, and polymer films in various thicknesses based on the electrode response were also investigated. The electrochemical behavior of DNA modified polymer electrode by using double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) was compared. DNA hybridization was also investigated. (C) 2009 Elsevier B.V. All rights reserved.