Purpose: The purpose of this study was to assess health-related physical fitness levels of Turkish kindergarten children and to show the differences of age a id gender. Material and methods: Sixteen kindergarten children were evaluated through 3 years from the beginning age of 34,62 +/- 5,6 months in this study. Health-related physical fitness tests were done in the 1st, 2nd and 3rd years, Each child completed a battery of health-related physical fitness tests including skinfold thickness for body composition, sit ups and pult-ups for muscular strength and endurance,. sit and reach test for flexibility, standing broad jump test for power, flamingo test for balance, and 10-meter running for speed and coordination. Results: Statistically significant improvements were seen In tests through years in all children (p<0.05) except for the sit-ups test (p>0.05), Girls had better results in flexibility anti balance tests (p<0.05), Conclusion: Girls have Otter results in flexibility all ages compared to boys, and physical fitness levels increase across ages in Turkish kindergarten children.