Immunomagnetic separation and a cultural reference method for detection of Salmonella spp. in foods

Mercanoglu B., Aytac S.

ARCHIV FUR LEBENSMITTELHYGIENE, vol.53, no.2, pp.43-45, 2002 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 53 Issue: 2
  • Publication Date: 2002
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.43-45
  • Hacettepe University Affiliated: No


An immunomagnetic separation (IMS)-core method that uses Dynabeads(R) anti-Salmonella and the ISO 6579 reference method were applied in order to isolate Salmonella spp. from sixty-five foodstuffs. The foodstuffs belonged to six different food groups that were poultry, raw meats, meat products, salads, traditional foods and eggs. Fifteen of the foodstuffs were spiked with S. enteritidis. As a result, IMS enabled the detection of 14 Salmonella positive samples from the 50 naturally contaminated samples examined whereas the ISO 6579 reference method yielded 12 Salmonella positive samples. In an evaluation using 15 spiked samples, this IMS-core method showed no difference in performance but was found to be more efficient than the ISO 6579 reference method in isolating Salmonella from a traditional food called garlic-flavoured sausageball. These results confirm that IMS is a rapid and highly sensitive method.