Establishment of primary myoblast cell cultures from cryopreserved skeletal muscle biopsies to serve as a tool in related research & development studies

Balci-Hayta, BURCU; Bekircan-Kurt, CAN; AKSU MENGEŞ, EVRİM; DAYANGAÇ ERDEN, DİDEM; Tan, Ersin; ERDEM ÖZDAMAR, SEVİM

doi:10.1016/j.jns.2018.08.018

Establishment of primary myoblast cell cultures from cryopreserved skeletal muscle biopsies to serve as a tool in related research & development studies

Copy For Citation

Balci-Hayta B., Bekircan-Kurt C. E., AKSU MENGEŞ E., DAYANGAÇ ERDEN D., Tan E., ERDEM ÖZDAMAR S.

JOURNAL OF THE NEUROLOGICAL SCIENCES, vol.393, pp.100-104, 2018 (SCI-Expanded)

Publication Type: Article / Article
Volume: 393
Publication Date: 2018
Doi Number: 10.1016/j.jns.2018.08.018
Journal Name: JOURNAL OF THE NEUROLOGICAL SCIENCES
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.100-104
Hacettepe University Affiliated: Yes

Abstract

Background: Primary myoblast cell cultures display the phenotypic characteristics and genetic defects of the donor tissue and represent an in vitro model system reflecting the disease pathology. They have been generated only from freshly harvested tissue biopsies. Here, we describe a novel technique to establish myoblast cell cultures from cryopreserved skeletal muscle biopsy tissues that are useful for diagnostic and research purposes.