Establishment of primary myoblast cell cultures from cryopreserved skeletal muscle biopsies to serve as a tool in related research & development studies


Balci-Hayta B., Bekircan-Kurt C. E. , AKSU MENGEŞ E. , DAYANGAÇ ERDEN D. , Tan E., ERDEM ÖZDAMAR S.

JOURNAL OF THE NEUROLOGICAL SCIENCES, cilt.393, ss.100-104, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 393
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.jns.2018.08.018
  • Dergi Adı: JOURNAL OF THE NEUROLOGICAL SCIENCES
  • Sayfa Sayıları: ss.100-104

Özet

Background: Primary myoblast cell cultures display the phenotypic characteristics and genetic defects of the donor tissue and represent an in vitro model system reflecting the disease pathology. They have been generated only from freshly harvested tissue biopsies. Here, we describe a novel technique to establish myoblast cell cultures from cryopreserved skeletal muscle biopsy tissues that are useful for diagnostic and research purposes.