Establishment of primary myoblast cell cultures from cryopreserved skeletal muscle biopsies to serve as a tool in related research & development studies

Balci-Hayta, BURCU; Bekircan-Kurt, CAN; AKSU MENGEŞ, EVRİM; DAYANGAÇ ERDEN, DİDEM; Tan, Ersin; ERDEM ÖZDAMAR, SEVİM

doi:10.1016/j.jns.2018.08.018

Establishment of primary myoblast cell cultures from cryopreserved skeletal muscle biopsies to serve as a tool in related research & development studies

Balci-Hayta B., Bekircan-Kurt C. E., AKSU MENGEŞ E., DAYANGAÇ ERDEN D., Tan E., ERDEM ÖZDAMAR S.

JOURNAL OF THE NEUROLOGICAL SCIENCES, cilt.393, ss.100-104, 2018 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 393
Basım Tarihi: 2018
Doi Numarası: 10.1016/j.jns.2018.08.018
Dergi Adı: JOURNAL OF THE NEUROLOGICAL SCIENCES
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.100-104
Hacettepe Üniversitesi Adresli: Evet

Özet

Background: Primary myoblast cell cultures display the phenotypic characteristics and genetic defects of the donor tissue and represent an in vitro model system reflecting the disease pathology. They have been generated only from freshly harvested tissue biopsies. Here, we describe a novel technique to establish myoblast cell cultures from cryopreserved skeletal muscle biopsy tissues that are useful for diagnostic and research purposes.