Establishment of primary myoblast cell cultures from cryopreserved skeletal muscle biopsies to serve as a tool in related research & development studies


Balci-Hayta B. , Bekircan-Kurt C. E. , AKSU MENGEŞ E. , DAYANGAÇ ERDEN D. , Tan E., ERDEM ÖZDAMAR S.

JOURNAL OF THE NEUROLOGICAL SCIENCES, vol.393, pp.100-104, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 393
  • Publication Date: 2018
  • Doi Number: 10.1016/j.jns.2018.08.018
  • Title of Journal : JOURNAL OF THE NEUROLOGICAL SCIENCES
  • Page Numbers: pp.100-104

Abstract

Background: Primary myoblast cell cultures display the phenotypic characteristics and genetic defects of the donor tissue and represent an in vitro model system reflecting the disease pathology. They have been generated only from freshly harvested tissue biopsies. Here, we describe a novel technique to establish myoblast cell cultures from cryopreserved skeletal muscle biopsy tissues that are useful for diagnostic and research purposes.