Research Information System
MEASUREMENT, 2025 (SCI-Expanded)
Although Baicalein (Ba) is the main bioactive flavonoid of Chinese medicinal herbs and has multiple pharmacological activities, it has unavoidably diverse side-effects. Thus, the quantification of Ba via interaction with double-stranded DNA (ds-DNA) molecule is crucial for assessing both its effectiveness and negative effects. In the study, new ds-DNA immobilized zinc oxide nanoparticles (ZnO NPs)-poly (cetyl trimethylammonium bromide), (p(CTAB)) modified pencil graphite electrode (PGE) was constructed to analyze Ba. The ZnO NPs-p(CTAB) composite was fabricated effortlessly, environmentally friendly and economically. The as-synthesized materials were characterized using scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDS), Fourier transform infrared spectroscopy (FTIR), electrochemical methods and thermogravimetric analysis (TGA). The fabricated platform was utilized for the quantification of ds-DNA and Ba for the first time. The created electrochemical sensor displayed favorable current response for sensing of ds-DNA with wide linear range (from 0.10 mu g mL- 1 to 100.0 mu g mL- 1) and low detection limit (LOD, 1.60 ng mL- 1) by differential pulse voltammetry. The offered sensing system was capable of measuring the Ba concentrations from 0.27 ng mL- 1 - 0.32 mu g mL- 1 with low LOD as 0.26 ng mL- 1. Moreover, the fabricated biosensor was tested for the monitoring of Ba in human bio-fluid with favorable recovery values between 97.0 to 104.0 %. Furthermore, a plausible interaction mechanism of ds-DNA and Ba was suggested as groove binding mode under the light of electrochemical and UV-vis spectrometry analyzes. All findings suggested that the created nano-biosensor system can become precious alternative to the existing approaches of Ba detection.