Reversible adsorption of catalase onto Fe3+ chelated poly(AAm-GMA)-IDA cryogels


MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol.50, pp.379-385, 2015 (SCI-Expanded) identifier identifier identifier


In this presented study, poly(acrylamide-glycidyl methacrylate) [poly(AAm-GMA)] cryogels were synthesized by cryopolymerization technique at sub-zero temperature. Prepared cryogels were then functionalized with iminodiacetic add (IDA) and chelated with Fe3+ ions in order produce the metal chelate affinity matrix. Synthesized cryogels were characterized with FTIR, ESEM and EDX analysis, and it was found that the cryogel had sponge like structure with interconnected pores and their pore diameter was about 200 mu m. Fe3+ chelated poly(AAm-GMA)-IDA cryogels were used for the adsorption of catalase and optimum adsorption conditions were determined by varying the medium pH, initial catalase concentration, temperature and ionic strength. Maximum catalase adsorption onto Fe3+ chelated poly(AAm-GMA)-IDA cryogel was found to be 12.99 mg/g cryogel at 25 degrees C, by using pH 5.0 acetate buffer. Adsorbed catalase was removed from the cryogel by using 1.0 M of NaCl solution and desorption yield was found to be 96%. Additionally, reusability profile of the Fe3+ chelated poly(AAm-GMA)-IDA cryogel was also investigated and it was found that, adsorption capacity of the cryogels didn't decrease significantly at the end of the 40 reuses. Catalase activity studies were also tested and it was demonstrated that desorbed catalase retained 70% of its initial activity. (C) 2015 Elsevier B.V. All rights reserved.