Investigation of phage and molasses interactions for the biocontrol of E. coli O157:H7

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Evran S., Tayyarcan E. K., Acar-Soykut E., GÜVEN B., DURAKLI VELİOĞLU S., BOYACI İ. H.

CANADIAN JOURNAL OF MICROBIOLOGY, cilt.68, sa.1, ss.55-65, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1139/cjm-2021-0211
  • Dergi Adı: CANADIAN JOURNAL OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.55-65
  • Anahtar Kelimeler: bacteriophage, phage therapy, synergism, molasses, MORUS-NIGRA L., ANTIMICROBIAL ACTIVITY, PHENOLIC-COMPOUNDS, IN-VITRO, MULBERRY, BACTERIOPHAGE, GROWTH, FOOD, EXTRACTS, ACIDS
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Resistance to antibiotics is one of the most critical health problems in the world. Therefore, finding new treatment methods to be used as alternatives to antibiotics has become a priority for researchers. Similar to phages, certain products containing antimicrobial components, such as molasses, are widely used to elimi-nate resistant bacteria. Molasses has a strong antimicrobial effect on bacterial cells, and this effect is thought to be due to the breakdown of the cytoplasmic cell membrane and cell proteins of the polyphenols in molas-ses. In the present study, phage-molasses interactions were investigated to examine the effects of concomi-tant use. It was found that molasses samples increased the size of phage plaques by up to 3-fold, and MIC and 1/2 x MIC concentrations of molasses increased the burst size of phages. Although no synergistic effect was found between the phage and molasses, the antimicrobial activities of the components and the effect of mo-lasses on phage activity were demonstrated.