Extraction and identification of proanthocyanidins from grape seed (Vitis Vinifera) using supercritical carbon dioxide


YILMAZ E. E., OZVURAL E. B., VURAL H.

JOURNAL OF SUPERCRITICAL FLUIDS, vol.55, no.3, pp.924-928, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 3
  • Publication Date: 2011
  • Doi Number: 10.1016/j.supflu.2010.10.046
  • Journal Name: JOURNAL OF SUPERCRITICAL FLUIDS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.924-928
  • Hacettepe University Affiliated: Yes

Abstract

In this study, supercritical carbon dioxide extraction of proantocyanidins (PRCs) was performed and the effect of different pressure, temperature and ethanol percentage was investigated. High performance liquid chromatography was used for the analysis of the compounds and it was found that the most effective parameter on the extraction was the amount of the ethanol percentage. Each compound was extracted from grape seeds at their maximum level when different parameters were used which was probably because of their different polarities. Gallic acid (GA), epigallocatechin (EGC) and epigallocatechingallate (EGCG) were extracted at their maximum level when the 300 bar 50 degrees C and 20% of ethanol was used. The maximum amount of catechin (CT) and epicatechin (ECT) were obtained when 300 bar 30 degrees C and 20% of ethanol was used for extraction, and 250 bar, 30 degrees C and 15% of ethanol was needed to extract highest amount of epicatechingallate (ECG). (C) 2010 Elsevier B.V. All rights reserved.