Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase


SOOMRO R., Percin I., MEMON N., BHANGER M. I., DENİZLİ A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.44, no.7, pp.1708-1713, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 7
  • Publication Date: 2016
  • Doi Number: 10.3109/21691401.2015.1089252
  • Journal Name: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1708-1713
  • Keywords: cryogel, gelatin, glycidyl methacrylate, horseradish peroxidase, phenol, ADSORPTION, REMOVAL, PHENOL, CATALASE, COMPOSITE, PROTEIN
  • Hacettepe University Affiliated: Yes

Abstract

Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. HRP immobilization capacity of p(HEMA-GMA)/gelatin cryogel discs was 24.8 mg/g. Removal of phenol from aqueous solutions was performed using HRP immobilized p(HEMA-GMA)/gelatin cryogel. It was observed that within 2 h of contact time, the percentage of phenol removal reaches up to 91% in the presence of H2O2.