Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.44, sa.7, ss.1708-1713, 2016 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 44 Sayı: 7
- Basım Tarihi: 2016
- Doi Numarası: 10.3109/21691401.2015.1089252
- Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1708-1713
- Anahtar Kelimeler: cryogel, gelatin, glycidyl methacrylate, horseradish peroxidase, phenol, ADSORPTION, REMOVAL, PHENOL, CATALASE, COMPOSITE, PROTEIN
- Hacettepe Üniversitesi Adresli: Evet
Özet
Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. HRP immobilization capacity of p(HEMA-GMA)/gelatin cryogel discs was 24.8 mg/g. Removal of phenol from aqueous solutions was performed using HRP immobilized p(HEMA-GMA)/gelatin cryogel. It was observed that within 2 h of contact time, the percentage of phenol removal reaches up to 91% in the presence of H2O2.