Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase

SOOMRO R., Percin I., MEMON N., BHANGER M. I., DENİZLİ A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.44, sa.7, ss.1708-1713, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 7
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3109/21691401.2015.1089252
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1708-1713
  • Anahtar Kelimeler: cryogel, gelatin, glycidyl methacrylate, horseradish peroxidase, phenol, ADSORPTION, REMOVAL, PHENOL, CATALASE, COMPOSITE, PROTEIN
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. HRP immobilization capacity of p(HEMA-GMA)/gelatin cryogel discs was 24.8 mg/g. Removal of phenol from aqueous solutions was performed using HRP immobilized p(HEMA-GMA)/gelatin cryogel. It was observed that within 2 h of contact time, the percentage of phenol removal reaches up to 91% in the presence of H2O2.