Molecularly Imprinted Polymers Based Surface Plasmon Resonance Sensor for Sulfamethoxazole Detection

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PHOTONIC SENSORS, vol.12, no.4, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 4
  • Publication Date: 2022
  • Doi Number: 10.1007/s13320-022-0658-5
  • Journal Name: PHOTONIC SENSORS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, INSPEC, Directory of Open Access Journals
  • Keywords: Antibiotic, sulfamethoxazole, nanofilm chip, molecularly imprinted polymers (MIP), surface plasmon resonance (SPR), STABILIZATION, NANOPARTICLES, ANTIBIOTICS, BIOSENSOR, SOIL, SPR
  • Hacettepe University Affiliated: Yes


Sulfamethoxazole (SMX) is a sulfonamide antibiotic primarily used to treat urinary tract infections and used in veterinary and industrialized husbandry to treat diseases and food additives. Like other antibiotics, SMX is considered as a pollutant in water and food that threaten local life. This study developed a surface plasmon resonance (SPR) sensor chip that is fast, highly selective, and reusable, and requires no pretreatment for detecting SMX. As a receptor, SMX imprinted methacrylic acid-2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate polymer [poly(MAA-HEMA-EGDMA)] was used. The surface of the gold SPR chips was coated with a drop-casting method. The nanofilm coated chips were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), ellipsometer, contact angle measurement, and Fourier-transform infrared spectrometry (FTIR). Imprinting factor (IF) was calculated as: Delta R[MIP(molecularly imprinted polymers)]/Delta R[NIP(non-imprinted)]=12/3.5=3.4. Limit of detection (LOD) and limit of quantification (LOQ) values were calculated with 3 s/m and 10 s/m methods, and the results were found to be 0.001 1 mu g/L for LOD 0.003 4 mu g/L for LOQ. Adsorption studies on both standard SMX solution and commercial milk samples were applied. Also, we investigated the developed chip's reusability, storability, and selectivity with amoxicillin and cefalexin.