8-OHdG sensing with MIP based solid phase extraction and QCM technique


Ersoez A., Diltemiz S. E. , Oezcan A. A. , DENİZLİ A. , SAY R.

SENSORS AND ACTUATORS B-CHEMICAL, cilt.137, ss.7-11, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 137 Konu: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.snb.2008.12.050
  • Dergi Adı: SENSORS AND ACTUATORS B-CHEMICAL
  • Sayfa Sayıları: ss.7-11

Özet

8-Hydroxy-2'-deoxyguanosine (8-OHdG) is one of the most abundant oxidative DNA lesions resulting from reactive oxygen species (ROS). The routine measurement of 8-OHdG in biologic samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. In this study, we have proposed an imprinting method for 8-OHdG recognition and determination by methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] as a new metal-chelating monomer via metal coordination-chelation interactions. The study includes the solid-phase extraction of blood samples by a new 8-OHdG imprinted sorbent and the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor via ligand interaction. 8-0HdG imprinted sorbent was prepared by bulk polymerization of MAAP-Fe(III) and N-N'-methylenebisacrylamide. By using a photo-graft surface polymerization technique with irradiation by ultra-violet (UV) light, a thin MIP film was prepared for the detection of 9-OHdG. The determined affinity constant (K alpha) were found as 78760 M-1 using 8-OHdG imprinted thin film. The 8-OHdG level in blood serum from a breast cancer patient was determined by the prepared QCM sensor. Lastly, analytical performances of this system was compared with capillary electrophoresis (CE) system. (c) 2008 Elsevier B.V. All rights reserved.