The aim of this study was to investigate the effect of static fatigue of trunk extensor and abdominal muscle groups on static and dynamic balance components. The study sample consisted of 40 healthy volunteers. Lumbar erector spinae, multifidus, and latissimus dorsi, external oblique, rectus abdominis and internal oblique muscles were measured on the dominant side. Dynamic and static balances were measured before and after the test protocol following EMG analysis. Pre-test and post-test static and dynamic balance scores showed that there was a statistically significant difference in OE (open-eyed) static balance values between the experimental and control groups (p<.01). There was a statistically significant difference in ATE (Average track error) scores between male and female participants (p<.05). There was a statistically significant difference in mean coordinated fatigue values of agonist and antagonist muscle groups in the Biering-Sorensen position between male and female participants (p<.05). Deterioration was observed in participants' OE static balance, indicating that OE static balance deterioration occurred again in the OE position. There was a statistically significant difference in co-fatigue values of abdominal muscles between the two groups.