Screening for free radical scavenging and cell aggregation inhibitory activities by secondary metabolites from Turkish Verbascum species


Tatli I. I. , Takamatsu S., Khan I. A. , Akdemir Z. S.

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, cilt.62, ss.673-678, 2007 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 62
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1515/znc-2007-9-1008
  • Dergi Adı: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Sayfa Sayıları: ss.673-678

Özet

Free radical scavenging and cell aggregation inhibitory activities of 36 secondary metabolites isolated from the methanolic extracts of Verbascum cilicicum Boiss., V lasianthum Boiss. ex Bentham, V pterocalycinum var. mutense Hub.-Mor., and V salviifolium Boiss. (Scrophulariaceae) were investigated. The isolated compounds, 6-O-vaniloyl ajugol (1), ilwensisaponin A (2), ilwensisaponin C (3), verbascoside (4),beta-hydroxyacteoside (5), martynoside (6), poliumoside (7), forsythoside B (8), angoroside A (9), dehydrodiconiferyl alcohol-9'-O-beta-D-glucopyranoside (10), dehydrodiconiferyl alcohol -9'-O-beta-D-gi ucopyranoside (11), apigenin 7-O-beta-pglucopyranoside (12), luteolin 7-O-beta-glucopyranoside (13), luteolin 3'-O-beta-glucopyranoside (14) and chrysoeriol 7-O-beta-glucopyranoside (15), exhibited a dose-dependent inhibition of bioautographic and spectrophotometric DPPH activities. Verbascoside (4) was the most active (IC50 4.0,mu g/ml) comparing it to vitamin C (IC50 4.4,mu g/ml) to inhibit phorbol 12-myristate 13-acetate (PMA)-induced peroxide-catalyzed oxidation of 2',7'-dichlorofluorescein (DCFH) by reactive oxygen species (ROS) within human promyelocytic HL-60 cells. Ilwensisaponin A (2) (MIC 6.9 mu g/ml) showed moderate in vitro activity on lymphocyte-associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1)-mediated aggregation using the HL-60 cell line [positive control was cytochalasin B (MlC 2.3,mu g/ml)]. None of the other compounds showed free radical scavenging and cell aggregation inhibitory activities.