In this study, chlorine from 2-chlorophenol was removed with crude laccase enzyme produced from Trametes versicolor ATCC (200801) grown in PDB culture including wheat straw. Effects of pH value, substrate concentration, enzyme amount, contact time and temperature on dechlorination were examined. Besides, dissolved oxygen consumption was followed simultaneously dechlorination and the decrease was observed. A change in toxicity of 2-chlorophenol under determined optimum conditions was monitored through Microtox. Also, after the dechlorination of 2-chlorophenol, changes in chemical structure of 2-chlorophenol was determined with FTIR analysis.