DECHLORINATION OF CHLORINE FROM 2-CHLOROPHENOL WITH TRAMETES VERSICOLOR CRUDE LACCASE

Akbulut, Merve; Aytar, Pinar; Gedikli, Serap; Unal, Arzu; ÇABUK, AHMET; Kolankaya, Nazif

DECHLORINATION OF CHLORINE FROM 2-CHLOROPHENOL WITH TRAMETES VERSICOLOR CRUDE LACCASE

Akbulut M., Aytar P., Gedikli S., Unal A., ÇABUK A., Kolankaya N.

SIGMA JOURNAL OF ENGINEERING AND NATURAL SCIENCES-SIGMA MUHENDISLIK VE FEN BILIMLERI DERGISI, cilt.30, sa.1, ss.9-19, 2012 (ESCI)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 30 Sayı: 1
Basım Tarihi: 2012
Dergi Adı: SIGMA JOURNAL OF ENGINEERING AND NATURAL SCIENCES-SIGMA MUHENDISLIK VE FEN BILIMLERI DERGISI
Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Academic Search Premier, Directory of Open Access Journals
Sayfa Sayıları: ss.9-19
Hacettepe Üniversitesi Adresli: Evet

Özet

In this study, chlorine from 2-chlorophenol was removed with crude laccase enzyme produced from Trametes versicolor ATCC (200801) grown in PDB culture including wheat straw. Effects of pH value, substrate concentration, enzyme amount, contact time and temperature on dechlorination were examined. Besides, dissolved oxygen consumption was followed simultaneously dechlorination and the decrease was observed. A change in toxicity of 2-chlorophenol under determined optimum conditions was monitored through Microtox. Also, after the dechlorination of 2-chlorophenol, changes in chemical structure of 2-chlorophenol was determined with FTIR analysis.