Germination response of three smoke- and karrikinolide-responsive species to gibberellic acid and reactive oxygen species

ÇATAV Ş. S., AKBAŞ K., TAVŞANOĞLU Ç., KÜÇÜKAKYÜZ K.

ANNALES BOTANICI FENNICI, sa.1, ss.31-40, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Dergi Adı: ANNALES BOTANICI FENNICI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, CAB Abstracts
  • Sayfa Sayıları: ss.31-40
  • Hacettepe Üniversitesi Adresli: Evet

Özet

There is a growing interest in understanding the mechanisms involved in seed germination stimulated by smoke and smoke-derived compounds. In this study, we aimed to determine the possible role of gibberellins (GAs) and reactive oxygen species (ROS) in the seed germination of three smoke-responsive and fire-adapted species, viz. Onopordum caricum (Asteraceae), Sarcopoterium spinosum (Rosaceae), and Stachys cretica (Lamiaceae). We conducted four germination experiments using smoke water, karrikinolide (KAR(1)), cyanohydrin mandelonitrile, gibberellic acid (GA(3)), paclobutrazol, hydrogen peroxide, and several agents generating ROS at different concentrations. The three species had a positive germination response to KAR(1). Sarcopoterium spinosum seeds were also sensitive to mandelonitrile. Exogenous GA(3) promoted the germination of all KAR(1)-responsive species in a concentration-specific way. Furthermore, GA biosynthesis was found to be required for the induction of germination by smoke water and KAR(1). Finally, ROS treatments stimulated the germination of KAR(1)- and cyanohydrin-sensitive S. spinosum seeds, but not only KAR(1)-sensitive O. caricum and S. cretica seeds. In conclusion, we showed that smoke-promoted germination is far more complex than previously believed and that future studies should be carried out using species with different sensitivities to smoke chemicals.