The aim of the study was to investigate the effect of individual room care in the neonatal intensive care unit (NICU) on the factors that influence mother-preterm infant interaction. Mothers in group I had hospitalization with their preterm infants in all individual room in the NICU. Mothers in group II were not hospitalized but had opportunity to visit their babies and spend time with them whenever they wanted. On the postdischarge third month, mothers were assessed for parental stress, postpartum depression, and perception of vulnerability. Although the mean depression, stress, and vulnerability scores were higher in group II, there was no significant difference between the groups (p > 0.05). Postpartum depression rate was more than double in group II, but this difference was not statistically, significant (p=0.06). Individual room care in the NICU cannot prevent maternal stress, postpartum depression, and perception of vulnerability, related to having a high-risk preterm infant by itself alone.