Superior resilience of non-axenic biogranules over axenic strains for robust biomineralization under environmental stress
International Journal of Environmental Science and Technology, cilt.23, sa.10, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 23 Sayı: 10
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s13762-026-07471-z
- Dergi Adı: International Journal of Environmental Science and Technology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, Environment Index, Geobase, INSPEC, Natural Science Collection (ProQuest), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Anahtar Kelimeler: Desiccation resilience, Environmental stressors, MICP, Pseudomonas aeruginosa, Sporosarcina pasteurii
- Hacettepe Üniversitesi Adresli: Evet
Özet
Microbially induced calcium carbonate precipitation (MICP) typically relies on axenic cultures and single metabolic pathways. Non-axenic cultures which can perform multiple microbial pathways and form resilient biofilms, are a promising alternative. Despite this, the superiority of non-axenic cultures’ MICP performance under varying environmental conditions remains insufficiently explored. This study evaluates the resilience of biogranules that can simultaneously conduct urea hydrolysis and nitrate reduction pathways under varying dissolved oxygen concentrations (anoxic to aerobic), temperatures (10–45 °C), and salinities (0.02–35 g.L−1 NaCl). In micronutrient-limited environment (0.05 g.L−1 yeast extract), biogranule performance was compared against well-studied ureolytic (Sporosarcina pasteurii), nitrate-reducing (Pseudomonas aeruginosa), and biogranule-derived isolates. Fresh biogranules achieved superior, consistent MICP performance across all stress conditions, maintaining complete calcium precipitation even when individual pathways were environmentally suppressed. Notably oven-dried biogranules successfully resuscitated and induced biomineralization, whereas desiccation stopped activity in all axenic cultures. S. pasteurii showed high short-term activity. Its performance was contingent on pre-synthesized enzymes and only 8% urea hydrolysis occurred when the culture was grown under anoxic and micronutrient scarce conditions. Biogranules showed robust metabolic compensation, where accelerated urea hydrolysis offset temperature induced declines in nitrate reduction. These findings highlight that the granular structure and microbial diversity provide protective niche and functional redundancy, offering a robust biomineralization performance.