An efficient approach that integrates a molecularly imprinted conducing polymer, polypyrrole (PPY) with a sensitive electrochemical impedance sensing platform for the quantification of dibutyl phthalate (DBP) was presented. The molecular imprinting process was employed by one-pot step by the electrochemical polymerization of the pyrrole which enabled control of polymer film thickness, easy adherence of the polymer layers on the sensing substrate and simplicity of the fabrication. Molecular imprinted polymer (MIP) modified surfaces were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). EIS technique was carried out as a detection method since DBP molecules are electrically insulative and non-electroactive. In the molecular imprinting process, each step was optimized and the linear response was obtained in the range between 0.01-1.0 mu M of DBP concentrations with a low detection limit as 4.5 nM under optimized conditions. Also, the relative standard deviation (RSD) was calculated as 5.21% for six different electrodes which were prepared independently. The developed sensor provided useful platform for rapid, simple and inexpensive detection of DBP.