Visual gamma stimulation causes prolonged enhancement of low-frequency blood flow oscillations across superficial cortical regions in mice

Bressan, Piergiulio; Long, Emily; Jiang, John; Vithayathil, Rebecca; Guan, Zhixuan; Song, Yuwen; Rauscher, Bradley; Chai, Nathan; Kılıç, Kıvılcım; Shah, Shashwat; ERDENER, ŞEFİK; Devor, Anna; Boas, David; Tang, Rockwell

doi:10.1177/0271678x261492627

Visual gamma stimulation causes prolonged enhancement of low-frequency blood flow oscillations across superficial cortical regions in mice

Bressan P. R., Long E., Jiang J., Vithayathil R., Guan Z., Song Y., ...Daha Fazla

Journal of Cerebral Blood Flow and Metabolism, 2026 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Basım Tarihi: 2026
Doi Numarası: 10.1177/0271678x261492627
Dergi Adı: Journal of Cerebral Blood Flow and Metabolism
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, MEDLINE, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Health Research Premium Collection (ProQuest), Pharma Collection (ProQuest)
Anahtar Kelimeler: blood flow, gamma entrainment, laser speckle, Low frequency oscillations, vasomotion
Hacettepe Üniversitesi Adresli: Evet

Özet

Introduction: Gamma entrainment using sensory stimuli (GENUS) uses 40 Hz-pulsed sensory stimuli to entrain neural activity in the gamma band (30-150 Hz). However, the effect of GENUS on low-frequency vascular oscillations has not been fully explored. Objectives: The objective of this study is to elucidate the effect of GENUS on vasomotion in healthy mice and potential confounds for future application in disease studies. Methods: Head-fixed, awake C57Bl/6 mice (n = 18; 9M and 9F) aged between 18 and 60 weeks were subjected to white light of either 40 Hz visual flicker (GENUS), or constant stimulus (Control). Blood flow was imaged using laser speckle contrast imaging (LSCI) before, during, immediately after 1 h of stimulus, and 30 min after the stimulus termination. Results: A linear mixed-effects model showed that GENUS enhanced the magnitude of 0.2-0.4 Hz blood flow oscillations by 38% during stimulation and by 30% at 30 min after stimulation compared to Control when controlled for age, sex, and other factors. The effect on vasomotion was distributed across the surface of many cortical regions not limited to visual areas. Conclusion: These results support the exploration of GENUS for increasing vasomotion in therapeutic contexts.