Flusilazole (C16H15F2N3Si) is a triazole fungicide and it is being used widely in recent years to control fungal infections in various fruits and vegetables. This study aims to evaluate the impact of flusilazole on cytotoxicity, ATP-dependent cassette transporter proteins (ABC transporter proteins) in SerW3 cells. In this study, SerW3 cells have administrated with 25, 100, and 200 mu M flusilazole, cell viability was performed. The quantity of the cellular lipids was evaluated spectrophotometrically. Moreover, the expression of the ABCA1 and ABCB1 proteins determined by immunofluorescence microscopy. Furtherly, evaluation of the cell death type and measurement of the activity of the antioxidant enzymes was performed. According to the results, flusilazole treatment gave rise to inhibition in cell viability, increase in apoptotic cell number, reduction in cellular lipids, and inhibition in the expression of ABCA1 and ABCB1 proteins. Furthermore, it caused decreases in antioxidant enzyme activities. It may be concluded that flusilazole administration may cause infertility/subfertility. The mechanism of action can be due to cytotoxicity, impairment of the detoxification mechanisms, lipid metabolism, and dysregulation of cell functions.