In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase

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Tandogan B., GÜVENÇ A., ÇALIŞ İ., Ulusu N. N.

ACTA BIOCHIMICA POLONICA, vol.58, no.4, pp.471-475, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 58 Issue: 4
  • Publication Date: 2011
  • Doi Number: 10.18388/abp.2011_2213
  • Journal Name: ACTA BIOCHIMICA POLONICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.471-475
  • Hacettepe University Affiliated: Yes

Abstract

Glutathione reductase (GR, E.C 1.6.4.2) is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.