Salinomycin encapsulated nanoparticles as a targeting vehicle for glioblastoma cells


TIĞLI AYDIN R. S. , Kaynak G., GÜMÜŞDERELİOĞLU M.

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, cilt.104, ss.455-464, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 104 Konu: 2
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/jbm.a.35591
  • Dergi Adı: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
  • Sayfa Sayıları: ss.455-464

Özet

Salinomycin has been introduced as a novel alternative to traditional anti-cancer drugs. The aim of this study was to test a strategy designed to deliver salinomycin to glioblastoma cells in vitro. Salinomycin-encapsulated polysorbate 80-coated poly(lactic-co-glycolic acid) nanoparticles (P80-SAL-PLGA) were prepared and characterized with respect to particle size, morphology, thermal properties, drug encapsulation efficiency and controlled salinomycin-release behaviour. The in vitro cellular uptake of P80-SAL-PLGA (5 and 10 mu M) or uncoated nanoparticles was assessed in T98G human glioblastoma cells, and the cell viability was investigated with respect to anti-growth activities. SAL, which was successfully transported to T98G glioblastoma cells via P80 coated nanoparticles (similar to 14% within 60 min), greatly decreased (p< 0.01) the cellular viability of T98G cells. Substantial morphological changes were observed in the T98G cells with damaged actin cytoskeleton. Thus, P80-SAL-PLGA nanoparticles induced cell death, suggesting a potential therapeutic role for this salinomycin delivery system in the treatment of human glioblastoma. (c) 2015 Wiley Periodicals, Inc.