beta-adrenergic Receptor Blocker ICI 118,551 Selectively Increases Intermediate-Conductance Calcium-Activated Potassium Channel (IKCa)-Mediated Relaxations in Rat Main Mesenteric Artery

ÖZKAN M. H. , Uma S.

BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, vol.122, no.6, pp.570-576, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 122 Issue: 6
  • Publication Date: 2018
  • Doi Number: 10.1111/bcpt.12949
  • Page Numbers: pp.570-576


Endothelial IKCa and/or SKCa channels play an important role in the control of vascular tone by participating in endothelium-dependent relaxation. Whether -AR antagonists, mainly used in hypertension, affect endothelial K-Ca channel function is unknown. In this study, we examined the effect of the 2-AR antagonist and inverse agonist ICI 118,551 on the IKCa/SKCa channel activity by assessing functional relaxation responses to several agonists that stimulate these channels. Mesenteric arterial rings isolated from male Sprague Dawley mounted to organ baths. Acetylcholine elicited IKCa- and SKCa-mediated relaxations that were abolished by TRAM-34 and apamin, respectively. ICI 118,551, which did not dilate the arteries per se, increased the IKCa-mediated relaxations, whereas SKCa-mediated relaxations remained unaltered. Same potentiating effect was also detected on the IKCa-mediated relaxations to carbachol and A23187, but not to NS309. Neither acetylcholine-induced nitric oxide-mediated relaxations nor SNP relaxations changed with ICI 118,551. The PKA inhibitor KT-5720, the selective 2-AR agonist salbutamol, the selective 2-AR antagonist butoxamine, the non-selective -AR antagonist propranolol, and the inverse agonists carvedilol or nadolol failed to affect the IKCa-mediated relaxations. ICI 118,551-induced increase was not reversed by salbutamol or propranolol as well. Besides, low potassium-induced relaxations in endothelium-removed arteries remained the same in the presence of ICI 118,551. These data demonstrate a previously unrecognized action of ICI 118,551, the ability to potentiate endothelial IKCa channel-mediated vasodilation, through a mechanism independent of 2-AR antagonistic or inverse agonistic action. Instead, the enhancement of acetylcholine relaxation seems likely to occur by a mechanism secondary to endothelial calcium increase.