Objectives: This study evaluated the effect of different root canal sealers on the push-out bond strength of tooth-colored posts to root dentin. Material and methods: Eighty human mandibular premolar teeth with single roots were decoronated and randomly divided into two groups according to post material: G1-G5: Cytec blanco; G6-G10: Cosmopost. In each group, the specimens were further subgrouped according to the filling material plus sealer (n=8): G1, G6: Gutta-percha+AH Plus; G2, G7: Resilon+Epiphany SE; G3, G8: Gutta-percha+Sealite; G4, G9: Gutta-percha+iRoot SP; and G5, G10: control (unobturated). Cytec blanco and Cosmopost of 1.4mm diameter were adhesively luted to samples using Variolink II. Push-out test was performed in a universal testing machine, and failure modes were examined under stereomicroscope. Data were analyzed with the two-way ANOVA and post hoc Tukey's tests. Statistical significance was set to 0.05. Results: Roots obturated with AH Plus (3.48 +/- 1.41MPa), Sealite (3.47 +/- 0.65MPa), and Resilon (3.36 +/- 1.23MPa) had the lowest bond strength (p<0.005). iRoot SP and control group samples showed the highest bond strength values (7.38 +/- 0.89MPa and 6.43 +/- 1.16MPa, respectively) (p<0.05). Significant differences were observed among tooth-colored posts and sealers (p<0.05). Adhesive failures were predominant in all groups (48%). Conclusions: When the resin cement Variolink II was used, the types of root canal filling materials and sealers could affect the retentions of the fiber/zirconium posts; the fiber post revealed the higher bond values than the zirconium post; and the calcium silicate-based sealer (iRoot SP) revealed the highest bond strengths.