Comparison of BBL Mycobacteria Growth Indicator Tube (MGIT) method, BACTEC radiometric system and Lowenstein-Jensen media for the detection of mycobacteria in clinical specimens Kli̇ni̇k örneklerde mi̇kobakteri̇leri̇n saptanmasinda BBL Mi̇kobakteri̇ Üreme İndi̇katör Tüp (MGIT) yöntemi̇, BACTEC radyometri̇k si̇stemi̇ ve Lowenstein-Jensen besi̇yeri̇ni̇n karşilaştirilmasi

ALP A., Hasçelik G.

Mikrobiyoloji Bulteni, cilt.36, sa.3-4, ss.229-235, 2002 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 3-4
  • Basım Tarihi: 2002
  • Dergi Adı: Mikrobiyoloji Bulteni
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.229-235
  • Anahtar Kelimeler: BACTEC 460 TB system, Lowenstein-Jensen medium, Mycobacteria, Mycobacteria Growth Indicator Tube(MGIT), Mycobacterium tuberculosis
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis. Several commercial systems, as well as conventional media, are available for the culture of acid-fast bacteria (AFB). The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processed clinical specimens. A total of 75 clinical specimens which were found positive for AFB in smear microscopy were included to the study, and of them 73 were grown in MGIT system, 70 in BACTEC 460 system and 74 on the LJ slants. Average periods for the detection of mycobacteria for BACTEC 460, MGIT systems and LJ medium were 7.3 days, 10.9 days and 19.4 days, respectively. As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system. It appears to be a safer system than BACTEC 460 system, and may be used for detection of mycobacteria in laboratories desiring a non-radioactive method.