Combination of Paclitaxel and R-flurbiprofen loaded PLGA nanoparticles suppresses glioblastoma growth on systemic administration

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Caban-Toktas S., Sahin A., Lule S., Esendagli G., Vural İ., Karlı Oguz K., ...Daha Fazla

International Journal of Pharmaceutics, cilt.578, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 578
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.ijpharm.2020.119076
  • Dergi Adı: International Journal of Pharmaceutics
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Glioma, PLGA, Nanoparticles, Paclitaxel, R-flurbiprofen, Nanomedicine, VITAMIN-E TPGS, POLY(LACTIC-CO-GLYCOLIC ACID) NANOPARTICLES, GLUTATHIONE-COATED NANOPARTICLES, BRAIN-TARGETED DELIVERY, COLON-CARCINOMA CELLS, CONTROLLED-RELEASE, IN-VITRO, SURFACE MODIFICATION, ANTITUMOR EFFICACY, CYCLE ARREST
  • Hacettepe Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier B.V.Malignant gliomas are highly lethal. Delivering chemotherapeutic drugs to the brain in sufficient concentration is the major limitation in their treatment due to the blood-brain barrier (BBB). Drug delivery systems may overcome this limitation and can improve the transportation through the BBB. Paclitaxel is an antimicrotubule agent with effective anticancer activity but limited BBB permeability. R-Flurbiprofen is a nonsteroidal antienflammatory drug and has potential anticancer activity. Accordingly, we designed an approach combining R-flurbiprofen and paclitaxel and positively-charged chitosan-modified poly-lactide-co-glycolic acid (PLGA) nanoparticles (NPs) and to transport them to glioma tissue. NPs were characterized and, cytotoxicity and cellular uptake studies were carried out in vitro. The in vivo efficacy of the combination and formulations were evaluated using a rat RG2 glioma tumor model. Polyethylene glycol (PEG) modified and chitosan-coated PLGA NPs demonstrated efficient cytotoxic activity and were internalized by the tumor cells in RG2 cell culture. In vivo studies showed that the chitosan-coated and PEGylated NPs loaded with paclitaxel and R-flurbiprofen exhibited significantly higher therapeutic activity against glioma. In conclusion, PLGA NPs can efficiently carry their payloads to glioma tissue and the combined use of anticancer and anti-inflammatory drugs may exert additional anti-tumor activity.