The effect of carvacrol in oleic acid induced acute lung injury in rats Siçanlarda karvakrol'un olei̇k asi̇tle oluşturulan akut akci̇ǧer hasarina etki̇si̇


Önal I. Ö., Erden I. A., AKINCI S. B., Erden G., KARABULUT İ., ZEYBEK N. D., ...More

Anestezi Dergisi, vol.19, no.2, pp.90-98, 2011 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 2
  • Publication Date: 2011
  • Journal Name: Anestezi Dergisi
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.90-98
  • Keywords: Antioxidants, Carvacrol, Oleic acid, SAcute lung injury
  • Hacettepe University Affiliated: Yes

Abstract

Objective: This study was conducted to compare the effects of oleic acid on the antioxidant systems of lung muscle tissue and carvacrol which has been proven to have healing effects on tumors of the lung, liver, breast, and testis and is known to have antioxidant effects, with respect to their healing and protective properties. Methods: 28 male rats of the Wistar type (250-350 grams) were included in the study. They were divided to four groups. Group I: Group Oleic acid (n: 7, 50 μl of oleic acid were administered intravenously (iv) and 30 minutes later 73 mg kg-1 of saline solution was given intraperitoneally (ip) as the same volume as carvacrol). Group II: Group Oleic acid+Carvacrol (n: 7, 50 μl of oleic acid was administered iv and 30 minutes later 73 mg kg-1 of carvacrol was administered ip). Group III: Group Control (n: 7, 50 μl of saline solution (the same dose as the oleic acid group) was administered and 30 minutes later 73 mg kg-1 of saline solution was administered ip). Group IV: Group Carvacrol: (n: 7, 50 μl of iv saline solution was administered and 30 minutes later 73 mg kg-1 carvacrol was administered ip). After being held in the oxygen room for 4 hours, blood and tissue samples were collected from the rats. Two histologists who were unaware of the group assignments assessed the acute lung injury via light microscopy; interstitial oedema, alveolar haemorrhage, intraalveolar neutrophils, intraalveolar macrophages and intraalveolar pneumocytes and electron microscopy; the integrity of the blood-gas barrier, the morphology of the type 1 and type 2 pneumocytes, and the presence of intraalveolar macrophages and granular material. The lung tissue was examined histopathologically and levels of protein carbonyl, malondialdehid tissue, catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) which are known as oxidant, anti-oxidant parameters were measured. Levels of antiinflammatory cytokines such as interleukin (IL)-6, IL-10, Tumor necrosis factor (TNF)-α and granulocyte colony stimulating factor (G-CSF) were measured in the blood samples. Results: Oleic acid caused significant total lung injury compared to the control group and to the vehicle group and levels of protein carbonyl and malondialdehid were higher than the other groups. Total lung tissue damage score, protein carbonyl, CAT, GSH-Px, SOD, levels were found to be similar in oleic acid and oleic acid+carvacrol groups. Levels of MDA tissue were lower in carvacrol and oleic acid+carvacrol groups than oleic acid group. Levels of proinflammatory and inflammatory cytokines IL-6, IL-10, TNF-α and G-CSF were found to be similar in both groups. Conclusion: As a result, it has been shown that because of the antioxidant effects of carvacrol in lung tissue, the increased use of carvacrol may be helpful in preventing and reversing lung tissue damage.