The estrogenic effects of apigenin, phloretin and myricetin based on uterotrophic assay in immature Wistar albino rats

BARLAS N., OZER S., Karabulut G.

TOXICOLOGY LETTERS, vol.226, no.1, pp.35-42, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 226 Issue: 1
  • Publication Date: 2014
  • Doi Number: 10.1016/j.toxlet.2014.01.030
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.35-42
  • Keywords: Phytoestrogen, Myricetin, Apigenin, Phloretin, Uterotrophic analysis, Rat, DIETARY PHYTOESTROGENS, OECD PROGRAM, FLAVONOIDS, MOUSE, VALIDATE, BIOASSAY, PHASE-2
  • Hacettepe University Affiliated: Yes


Chemicals that occur in vegetal food and known as phytoestrogens, because of their structures similarity to estrogen, have benefits on chronic diseases. Despite this, when they are taken at high amounts, they can cause harmful effects on endocrine system of human and animals. In this study, it has been intended to determine the estrogenic potencies of phytoestrogens apigenin, phloretin and myricetin whose affinities for estrogen receptors in vitro. The female rats divided into 17 groups, each containing six rats. There was a negative control group and there were positive control dose groups which contains ethinyl estradiol, ethinyl estradiol + tamoxifen and genistein. The other dose groups which were tested for estrogenic activity contains apigenin, myricetin and phloretin All chemicals have been given to Wistar immature female rats with oral gavage for 3 consecutive days. By using uterotrophic analysis, uterus wet and blotted weights, vaginal opening, uterus length of female rats has been recorded at the end of the experiment. For detect of cell response, luminal epithelium height, gland number and lactoferrin intensity in luminal epithelium of uterus were evaluated. Biochemical analysises in blood were performed.