Exploring the Binding Affinity of Newly Synthesized N-Tosylated Carbazole Derivatives With HSA/Lysozyme by Spectral and Computational Analysis
ChemistrySelect, cilt.11, sa.26, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 11 Sayı: 26
- Basım Tarihi: 2026
- Doi Numarası: 10.1002/slct.73815
- Dergi Adı: ChemistrySelect
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Academic Search Ultimate (EBSCO)
- Anahtar Kelimeler: 3D fluorescence, carbazole, FRET, HSA, lysozyme, molecular docking
- Hacettepe Üniversitesi Adresli: Evet
Özet
The two new N-tosylated carbazole derivatives (CTCl and CTMe) were synthesized and their binding properties with the two proteins (HSA and lysozyme) have been examined by UV absorption, fluorescence (steady-state and 3D) spectroscopies and computational methods. Fluorescence measurements revealed that the derivatives quenched the fluorescence of the proteins via static mechanism. The positive signs of both ΔS and ΔH parameters for the binding affinity between the derivatives and proteins indicated the presence of hydrophobic interactions. Three-dimensional fluorescence spectra showed that binding of the derivatives to proteins caused conformational changes in the protein molecule. The molecular docking results were consistent with the experimental results and confirmed the types of bonds formed between CTCl/CTMe and proteins.