Preparation and characterization of triamcinolone acetonide-loaded poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHx) microspheres

BAYRAM C., DENKBAŞ E. B., Kilicay E., Hazer B., CAKMAK H. B., NODA I.

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, vol.23, no.4, pp.334-347, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 4
  • Publication Date: 2008
  • Doi Number: 10.1177/0883911508092790
  • Journal Name: JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.334-347
  • Keywords: triamcinolone acetonide, PHBHx, cystoid macular oedema, uveitis, microspheres, controlled drug delivery, controlled drug release, CYSTOID MACULAR EDEMA, INTRAVITREAL TRIAMCINOLONE, DELIVERY, DEGENERATION, PERSPECTIVES, INJECTIONS, RETINITIS, THERAPY, IMPLANT
  • Hacettepe University Affiliated: Yes

Abstract

Triamcinolone acetonide loaded in poly(3-hydroxybutyrate-co-3 hydroxyhexanoate) (PHBHx) microspheres were prepared to treat cystoid macular oedema (CMO) and acute posterior segment inflammation associated with uveitis. The PHBHx microspheres were prepared by solvent evaporation technique using methylene chloride as the solvent and aqueous poly(vinyl alcohol) emulsifier as the dispersion medium. The PHBHx microspheres obtained were well formed with narrow size distribution; the average size prepared ranged from 40-200 mu m depending on the formulation used. The stirring rate of the dispersion medium, emulsifier concentration, and polymer/solvent ratio parameters were varied to determine their effect on the size and size distribution of the PHBHx microspheres. Increasing the stirring rate and emulsifier concentration decreased the size and the size distribution of the microspheres, while increasing the polymer/solvent ratio caused the opposite effect. The polymer/drug ratio was the most effective parameter for controlling drug loading and release properties. More than 90% of the loaded drug was released within the first 24 h; after that, the release rate was slower for all formulations.