A Simple Extraction Procedure for HPLC Analysis of Fluoxetine in Rat Plasma Samples


REÇBER T., Ozkan E., Eren-Kocak E., YILMAZ M., NEMUTLU E., KIR S.

CURRENT PHARMACEUTICAL ANALYSIS, vol.13, no.1, pp.80-84, 2017 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 1
  • Publication Date: 2017
  • Doi Number: 10.2174/1573412912666160422153616
  • Journal Name: CURRENT PHARMACEUTICAL ANALYSIS
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.80-84
  • Keywords: Fluoxetine, high performance liquid chromatography, rat plasma, validation, fluorescence detection, PERFORMANCE LIQUID-CHROMATOGRAPHY, ACTIVE METABOLITE NORFLUOXETINE, ELECTRON-CAPTURE DETECTION, TANDEM MASS-SPECTROMETRY, DIODE-ARRAY DETECTION, GAS-CHROMATOGRAPHY, FLUORESCENCE DETECTION, PRECOLUMN DERIVATIZATION, ANTIDEPRESSANT DRUGS, HUMAN URINE

Abstract

In present study, a simple liquid-liquid extraction-high performance liquid chromatography-fluorescence method was developed for the determination of fluoxetine (FLU) in rat plasma samples. A MZ Analytical Column C-18 (250 mm x 4.6 mm, 5 mu m i.d.) was used for the separation of FLU. The total analysis time was less than 7 minutes. The rat plasma samples were simply treated with acetonitrile and analyzed using developed method. The recovery of the FLU from rat plasma samples is 92.70% +/- 0.48. FLU in spiked rat plasma samples provided linear data over a range of 0.2 - 2.00 mu g mL(-1). The method was validated according to the FDA guidelines and can be proposed for quantification of FLU in rat plasma.