In present study, a simple liquid-liquid extraction-high performance liquid chromatography-fluorescence method was developed for the determination of fluoxetine (FLU) in rat plasma samples. A MZ Analytical Column C-18 (250 mm x 4.6 mm, 5 mu m i.d.) was used for the separation of FLU. The total analysis time was less than 7 minutes. The rat plasma samples were simply treated with acetonitrile and analyzed using developed method. The recovery of the FLU from rat plasma samples is 92.70% +/- 0.48. FLU in spiked rat plasma samples provided linear data over a range of 0.2 - 2.00 mu g mL(-1). The method was validated according to the FDA guidelines and can be proposed for quantification of FLU in rat plasma.